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saci restriction enzymes  (New England Biolabs)


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    Structured Review

    New England Biolabs saci restriction enzymes
    Saci Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1096 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/saci+restriction+enzymes/SacI/pmc13001215-325-9-12
    Average 96 stars, based on 1096 article reviews
    saci restriction enzymes - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Expression of SPAG7 and its regulatory microRNAs in seminal plasma and seminal plasma-derived extracellular vesicles of patients with subfertility
    Article Snippet: The synthesized fragments were delivered as inserts and the restriction enzymes EcoRI and BamHI (New England Biolabs) were used to subclone the fragments into the pSG5 vector. .. In addition, the 3’UTR of SPAG7 was cloned into the pMIR-RNL-TK dual-luciferase reporter gene vector (Thermo Fisher Scientific), downstream to the luciferase gene using SpeI and SacI restriction enzymes (New England Biolabs). ..

    Article Title: Cryptochrome 1 Ubiquitously Regulates Hypocotyl Elongation from Arabidopsis to Sacred Lotus (Nelumbo nucifera)
    Article Snippet: Cryptochromes (CRYs) are vital regulators of blue light-dependent photoresponses, and multiple CRYs were characterized in basal eudicot-sacred lotus (Nelumbo nucifera).. However, how blue light senses and regulates photomorphogenesis in sacred lotus remains unexplored.. In this study, we found that sacred lotus Cryptochrome 1a (NnCRY1a) regulated photomorphogenesis in a blue light-dependent manner.

    Plasmid Preparation:

    Article Title: Expression of SPAG7 and its regulatory microRNAs in seminal plasma and seminal plasma-derived extracellular vesicles of patients with subfertility
    Article Snippet: The synthesized fragments were delivered as inserts and the restriction enzymes EcoRI and BamHI (New England Biolabs) were used to subclone the fragments into the pSG5 vector. .. In addition, the 3’UTR of SPAG7 was cloned into the pMIR-RNL-TK dual-luciferase reporter gene vector (Thermo Fisher Scientific), downstream to the luciferase gene using SpeI and SacI restriction enzymes (New England Biolabs). ..

    Article Title: Multi-omics reveals DEAD-box RNA helicase 20 as key protein enhancing wilt resistance in transgenic chickpea.
    Article Snippet: Key message AtNPR1 expression strengthens Fusarium wilt resistance in chickpea by activating SAR.. Multi-omics analyses suggest CaDEAD-box20 as a candidate gene contributing to resistance through possible interaction with AtNPR1.. Abstract Traditional breeding for broad-spectrum disease resistance in crops is often slow and resource-intensive, whereas genetic engineering provides a more precise and efficient alternative.

    Article Title: Cryptochrome 1 Ubiquitously Regulates Hypocotyl Elongation from Arabidopsis to Sacred Lotus (Nelumbo nucifera)
    Article Snippet: Cryptochromes (CRYs) are vital regulators of blue light-dependent photoresponses, and multiple CRYs were characterized in basal eudicot-sacred lotus (Nelumbo nucifera).. However, how blue light senses and regulates photomorphogenesis in sacred lotus remains unexplored.. In this study, we found that sacred lotus Cryptochrome 1a (NnCRY1a) regulated photomorphogenesis in a blue light-dependent manner.

    Luciferase:

    Article Title: Expression of SPAG7 and its regulatory microRNAs in seminal plasma and seminal plasma-derived extracellular vesicles of patients with subfertility
    Article Snippet: The synthesized fragments were delivered as inserts and the restriction enzymes EcoRI and BamHI (New England Biolabs) were used to subclone the fragments into the pSG5 vector. .. In addition, the 3’UTR of SPAG7 was cloned into the pMIR-RNL-TK dual-luciferase reporter gene vector (Thermo Fisher Scientific), downstream to the luciferase gene using SpeI and SacI restriction enzymes (New England Biolabs). ..

    Expressing:

    Article Title: Plant-produced monoclonal antibodies recognize the native circumsporozoite protein on Plasmodium falciparum sporozoites
    Article Snippet: The variable regions of the HC and LC were commercially synthesized (Genewiz, Suzhou, China) and fused to the constant domains of the human IgG1 gamma heavy chain and kappa light chain, respectively. .. The plant-optimized HC and LC sequences were enzymatically digested with XbaI and SacI restriction enzymes (New England BioLabs, USA) and ligated into the pBY2ek geminiviral expression vector. ..



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